Hotspot

accD-psaI

LSC · 63076-63885 · 810 bp

Back to Bombycidendron

Pi 0.0213

MarkerSeek score 65.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 16
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTAGTTCAGCTCCACGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	899-937 bp	1.0	85.2
accD-psaI_p2	AGTTCAGCTCCACGGTTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	894-932 bp	1.0	84.8
accD-psaI_p3	GAGTTAGTTCAGCTCCACGG	GAGGGAAAGCTGCTGAAAGT	55.0	50.0	58.0	58.1	852-890 bp	1.0	84.7
accD-psaI_p4	GAGTTAGTTCAGCTCCACGG	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	905-943 bp	1.0	84.5
accD-psaI_p5	AGTTCAGCTCCACGGTTTTT	GAGGGAAAGCTGCTGAAAGT	45.0	50.0	57.9	58.1	847-885 bp	1.0	84.3