Hotspot

trnT-GGU-psbD

LSC · 33729-35229 · 1501 bp

Back to Bombycidendron

Pi 0.00225

MarkerSeek score 63.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1501
variable_sites: 3
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1503-1643 bp	1.0	83.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1506-1646 bp	1.0	83.4
trnT-GGU-psbD_p3	ACGGCCCTTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1520-1660 bp	1.0	83.3
trnT-GGU-psbD_p4	AATTGAATCGAACGGCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.2	58.0	1531-1671 bp	1.0	82.8
trnT-GGU-psbD_p5	ATTGAATCGAACGGCCCTTT	GAAACGGTCCCTACGTAACC	45.0	55.0	58.2	58.0	1530-1670 bp	1.0	82.8