Hotspot

trnK-UUU-rps16

LSC · 4587-5538 · 952 bp

Back to Bombycidendron

Pi 0.00662

MarkerSeek score 67.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 6
pi: 0.00662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1035-1080 bp	1.0	82.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1034-1079 bp	1.0	82.4
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	1021-1066 bp	1.0	80.1
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	995-1040 bp	1.0	79.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	994-1039 bp	1.0	79.7