Hotspot

petD

LSC · 81422-82679 · 1258 bp

Pi 0

MarkerSeek score 61.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1258
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TTCCCCGAAGAGAAAATGGA	ACTCCCTAGATACGCACATCA	45.0	47.6	56.4	58.1	1285-1324 bp	1.0	61.5
petD_p2	TCCCCGAAGAGAAAATGGAT	ACTCCCTAGATACGCACATCA	45.0	47.6	56.2	58.1	1284-1323 bp	1.0	59.6
petD_p3	ATTCCCCGAAGAGAAAATGGA	ACTCCCTAGATACGCACATCA	42.9	47.6	57.0	58.1	1286-1325 bp	1.0	57.8
petD_p4	TCCCCGAAGAGAAAATGGATT	ACTCCCTAGATACGCACATCA	42.9	47.6	57.0	58.1	1284-1323 bp	1.0	57.8
petD_p5	TTCCCCGAAGAGAAAATGGAT	ACTCCCTAGATACGCACATCA	42.9	47.6	57.0	58.1	1285-1324 bp	1.0	57.8