Hotspot

atpF

LSC · 12265-13641 · 1377 bp

Back to Bombax

Pi 0.000726
MarkerSeek score 59.20
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1377
variable_sites
1
pi
0.000726
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 CGTCGGCTCTAATAGTTACCA GTCTATCTAAAATAAAATAGGAGATCA 47.6 25.9 57.0 53.1 1504 bp 1.0 40.1
atpF_p2 CGTCGGCTCTAATAGTTACCA AGTCTATCTAAAATAAAATAGGAGATC 47.6 25.9 57.0 52.8 1505 bp 1.0 40.1
atpF_p3 TCGTCGGCTCTAATAGTTACC GTCTATCTAAAATAAAATAGGAGATCA 47.6 25.9 56.7 53.1 1505 bp 1.0 40.1
atpF_p4 TCGTCGGCTCTAATAGTTACCA GTCTATCTAAAATAAAATAGGAGATCA 45.5 25.9 58.5 53.1 1505 bp 1.0 40.1
atpF_p5 TCGTCGGCTCTAATAGTTACC AGTCTATCTAAAATAAAATAGGAGATC 47.6 25.9 56.7 52.8 1506 bp 1.0 40.1