Hotspot

rpl32-ndhF

SSC · 131696-132930 · 1235 bp

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Pi 0.0461
MarkerSeek score 88.40
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1235
variable_sites
55
pi
0.0461
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 AATGCCGCCCAATATCCTTT GGGAAGTGGCACAAAAGGTA 45.0 50.0 57.9 58.0 1364-1372 bp 1.0 84.9
rpl32-ndhF_p2 ATGCCGCCCAATATCCTTTT GGGAAGTGGCACAAAAGGTA 45.0 50.0 57.9 58.0 1363-1371 bp 1.0 84.9
rpl32-ndhF_p3 TGCCGCCCAATATCCTTTTT GGGAAGTGGCACAAAAGGTA 45.0 50.0 58.1 58.0 1362-1370 bp 1.0 84.5
rpl32-ndhF_p4 AATGCCGCCCAATATCCTTT AAAGGAATGGGAAGTGGCAC 45.0 50.0 57.9 58.4 1372-1380 bp 1.0 82.0
rpl32-ndhF_p5 ATGCCGCCCAATATCCTTTT AAAGGAATGGGAAGTGGCAC 45.0 50.0 57.9 58.4 1371-1379 bp 1.0 82.0