Hotspot

atpH-atpI

LSC · 14519-15758 · 1240 bp

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Pi 0
MarkerSeek score 61.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1240
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.0 57.9 1331-1361 bp 1.0 80.5
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.0 57.9 1297-1327 bp 1.0 80.5
atpH-atpI_p3 TACCTTCTACAGCTTGGCCT TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.0 57.9 1380-1410 bp 1.0 80.3
atpH-atpI_p4 AATAGAAGCAAGCCCGACAG CGAATCCATGGAGGGTCATC 50.0 55.0 58.0 57.8 1301-1331 bp 1.0 80.2
atpH-atpI_p5 ATAACGGAAGCGGCAGAAAT CGAATCCATGGAGGGTCATC 45.0 55.0 58.0 57.8 1267-1297 bp 1.0 80.2