Hotspot

atpF-atpH

LSC · 13642-14272 · 631 bp

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Pi 0
MarkerSeek score 59.60
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
631
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG TGGTTGTAGCATTGGCACTT 50.0 45.0 57.9 58.0 622-744 bp 1.0 83.0
atpF-atpH_p2 CCAGTAACCCAAGGAAACGA TGGTTGTAGCATTGGCACTT 50.0 45.0 57.4 58.0 595-717 bp 1.0 79.5
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG CTGGTTGTAGCATTGGCACT 50.0 50.0 57.9 58.5 623-745 bp 1.0 79.4
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG ATGGGCTGGTTGTAGCATTG 50.0 50.0 57.9 58.5 628-750 bp 1.0 78.9
atpF-atpH_p5 GGCCAGTAACCCAAGGAAAC TGGTTGTAGCATTGGCACTT 55.0 45.0 58.7 58.0 597-719 bp 1.0 78.0