Hotspot

trnS-GCU-trnG-GCC

LSC · 8616-9529 · 914 bp

Back to Bombax

Pi 0
MarkerSeek score 61.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
914
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ATGAATCAAACCGAGGGACC 50.0 50.0 58.0 57.6 905-1029 bp 1.0 80.6
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ATGAATCAAACCGAGGGACC 50.0 50.0 58.0 57.6 904-1028 bp 1.0 80.6
trnS-GCU-trnG-GCC_p3 CGCACAACGGATTAGCAATC ATGAATCAAACCGAGGGACC 50.0 50.0 57.9 57.6 920-1044 bp 1.0 79.9
trnS-GCU-trnG-GCC_p4 ACGGAAAGAGAGGGATTCGA ATGAATCAAACCGAGGGACC 50.0 50.0 58.2 57.6 959-1083 bp 1.0 79.4
trnS-GCU-trnG-GCC_p5 TCGCACAACGGATTAGCAAT ATGAATCAAACCGAGGGACC 45.0 50.0 58.3 57.6 921-1045 bp 1.0 78.5