Hotspot

trnK-UUU-rps16

LSC · 4722-5565 · 844 bp

Back to Bombax

Pi 0.00246
MarkerSeek score 63.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
844
variable_sites
2
pi
0.00246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 972-1005 bp 1.0 81.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 971-1004 bp 1.0 81.3
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.5 58.0 958-991 bp 1.0 79.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 932-965 bp 1.0 78.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAGGCGGGGGTTTTTAC 50.0 50.0 58.2 58.4 931-964 bp 1.0 78.5