primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	7.6	0.0	0.895799	1851	2132	429-429	1.000000	72.8
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	0.0	0.0	0.944750	1851	2132	405-405	1.000000	72.4
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	AACACCAAACCATCCGATGT	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.7	58.8	7.6	0.0	1.114189	1851	2132	419-419	1.000000	71.1
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	AACACCAAACCATCCGATGT	GACTAGTTCCGGGTTCGAGT	20	20	45.0	55.0	57.7	58.8	7.6	0.0	1.114189	1851	2132	420-420	1.000000	71.1
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	ACGGTTTTCAGTGCTGGTTA	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.7	58.8	0.0	0.0	1.163141	1851	2132	395-395	1.000000	70.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4722	5565	972-1005	1.000000	81.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4722	5565	971-1004	1.000000	81.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	57.5	58.0	0.0	17.2	0.473875	4722	5565	958-991	1.000000	79.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4722	5565	932-965	1.000000	78.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4722	5565	931-964	1.000000	78.5
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.424641	8616	9529	905-1029	1.000000	80.6
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.426029	8616	9529	904-1028	1.000000	80.6
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.511210	8616	9529	920-1044	1.000000	79.9
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.571407	8616	9529	959-1083	1.000000	79.4
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	20	20	45.0	50.0	58.3	57.6	0.0	0.0	0.687118	8616	9529	921-1045	1.000000	78.5
atpF_p1	atpF	1	CGTCGGCTCTAATAGTTACCA	GTCTATCTAAAATAAAATAGGAGATCA	21	27	47.6	25.9	57.0	53.1	0.0	0.0	13.907880	12265	13641	1504-1504	1.000000	40.1
atpF_p2	atpF	2	CGTCGGCTCTAATAGTTACCA	AGTCTATCTAAAATAAAATAGGAGATC	21	27	47.6	25.9	57.0	52.8	0.0	0.0	14.146104	12265	13641	1505-1505	1.000000	40.1
atpF_p3	atpF	3	TCGTCGGCTCTAATAGTTACC	GTCTATCTAAAATAAAATAGGAGATCA	21	27	47.6	25.9	56.7	53.1	0.0	0.0	14.181943	12265	13641	1505-1505	1.000000	40.1
atpF_p4	atpF	4	TCGTCGGCTCTAATAGTTACCA	GTCTATCTAAAATAAAATAGGAGATCA	22	27	45.5	25.9	58.5	53.1	0.0	0.0	14.386403	12265	13641	1505-1505	1.000000	40.1
atpF_p5	atpF	5	TCGTCGGCTCTAATAGTTACC	AGTCTATCTAAAATAAAATAGGAGATC	21	27	47.6	25.9	56.7	52.8	0.0	0.0	14.420167	12265	13641	1506-1506	1.000000	40.1
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124357	13642	14272	622-744	1.000000	83.0
atpF-atpH_p2	atpF-atpH	2	CCAGTAACCCAAGGAAACGA	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	57.4	58.0	0.0	0.0	0.560414	13642	14272	595-717	1.000000	79.5
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.580266	13642	14272	623-745	1.000000	79.4
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	ATGGGCTGGTTGTAGCATTG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.640760	13642	14272	628-750	1.000000	78.9
atpF-atpH_p5	atpF-atpH	5	GGCCAGTAACCCAAGGAAAC	TGGTTGTAGCATTGGCACTT	20	20	55.0	45.0	58.7	58.0	0.0	0.0	0.752018	13642	14272	597-719	1.000000	78.0
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14519	15758	1331-1361	1.000000	80.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14519	15758	1297-1327	1.000000	80.5
atpH-atpI_p3	atpH-atpI	3	TACCTTCTACAGCTTGGCCT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	3.3	0.0	0.180119	14519	15758	1380-1410	1.000000	80.3
atpH-atpI_p4	atpH-atpI	4	AATAGAAGCAAGCCCGACAG	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	58.0	57.8	0.0	21.3	0.205716	14519	15758	1301-1331	1.000000	80.2
atpH-atpI_p5	atpH-atpI	5	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	14519	15758	1267-1297	1.000000	80.2
rpoC1_p1	rpoC1	1	GGTCCTAGTTGAACCGGAGA	TTGCTCGTCCGAGAATTACG	20	20	55.0	50.0	58.4	58.1	9.5	20.1	0.542150	22000	24823	3000-3000	1.000000	75.7
rpoC1_p2	rpoC1	2	TAGTTGAACCGGAGAGACCC	TTGCTCGTCCGAGAATTACG	20	20	55.0	50.0	58.4	58.1	9.5	20.1	0.542150	22000	24823	2995-2995	1.000000	75.7
rpoC1_p3	rpoC1	3	GGTCCTAGTTGAACCGGAGA	ACGATCTTTGGCTCTGGAAC	20	20	55.0	50.0	58.4	57.9	9.5	0.0	0.546898	22000	24823	2983-2983	1.000000	75.7
rpoC1_p4	rpoC1	4	TAGTTGAACCGGAGAGACCC	ACGATCTTTGGCTCTGGAAC	20	20	55.0	50.0	58.4	57.9	9.5	0.0	0.546898	22000	24823	2978-2978	1.000000	75.7
rpoC1_p5	rpoC1	5	TAGTTGAACCGGAGAGACCC	CGATCTTTGGCTCTGGAACT	20	20	55.0	50.0	58.4	57.6	9.5	0.0	0.839565	22000	24823	2977-2977	1.000000	73.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32554	33394	908-928	1.000000	80.5
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	32554	33394	906-926	1.000000	80.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32554	33394	962-982	1.000000	80.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32554	33394	887-907	1.000000	80.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	32554	33394	885-905	1.000000	80.1
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TGGTTAAAGATCACGAGGCG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.119925	44499	45414	996-1026	1.000000	81.4
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	44499	45414	1064-1094	1.000000	81.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	44499	45414	1046-1076	1.000000	81.0
psaA-ycf3_p4	psaA-ycf3	4	TTCTTGAGAAATGACCCGGC	TGGTTAAAGATCACGAGGCG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.183366	44499	45414	1019-1049	1.000000	80.9
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TAAAGATCACGAGGCGCTTC	20	20	50.0	50.0	57.9	58.1	4.1	9.9	0.192772	44499	45414	992-1022	1.000000	80.8
trnL-UAA_p1	trnL-UAA	1	AGAAAGGGGAAAGGAAGGAGA	ATAGCAAACGGGCTTTTTGG	21	20	47.6	45.0	58.0	57.3	0.0	3.8	1.747288	50548	51211	735-761	1.000000	68.8
trnL-UAA_p2	trnL-UAA	2	AGGGGAAAGGAAGGAGAACG	ATAGCAAACGGGCTTTTTGG	20	20	55.0	45.0	59.0	57.3	0.0	3.8	1.756764	50548	51211	731-757	1.000000	68.7
trnL-UAA_p3	trnL-UAA	3	AGGGCATCCTAATGAGATCCT	ATAGCAAACGGGCTTTTTGG	21	20	47.6	45.0	57.7	57.3	0.0	3.8	2.030213	50548	51211	702-728	1.000000	66.7
trnL-UAA_p4	trnL-UAA	4	AAGGGGAAAGGAAGGAGAAC	ATAGCAAACGGGCTTTTTGG	20	20	50.0	45.0	56.7	57.3	0.0	3.8	2.019696	50548	51211	732-758	1.000000	66.6
trnL-UAA_p5	trnL-UAA	5	AAAGGGGAAAGGAAGGAGAAC	ATAGCAAACGGGCTTTTTGG	21	20	47.6	45.0	57.5	57.3	0.0	3.8	2.269266	50548	51211	733-759	1.000000	64.6
trnV-UAC_p1	trnV-UAC	1	TACTCTGAGCGATCTCCCTG	AGAGGCAAACAATTGAGGCA	20	20	55.0	45.0	58.0	58.0	4.2	13.6	0.026087	55018	55707	1502-1531	1.000000	81.3
trnV-UAC_p2	trnV-UAC	2	TACTCTGAGCGATCTCCCTG	CGAGTAGAGGCTATCAACGC	20	20	55.0	55.0	58.0	58.0	4.2	0.0	0.065062	55018	55707	1455-1484	1.000000	81.1
trnV-UAC_p3	trnV-UAC	3	TACTCTGAGCGATCTCCCTG	CTCTGATGGGTGGTTTTGCT	20	20	55.0	50.0	58.0	58.1	4.2	0.0	0.110594	55018	55707	1646-1675	1.000000	80.5
trnV-UAC_p4	trnV-UAC	4	TACTCTGAGCGATCTCCCTG	ACTTGAGGAAAGCTGAAGGC	20	20	55.0	50.0	58.0	58.1	4.2	0.0	0.122797	55018	55707	1523-1552	1.000000	80.5
trnV-UAC_p5	trnV-UAC	5	TACTCTGAGCGATCTCCCTG	TGAGGAAAGCTGAAGGCAAG	20	20	55.0	50.0	58.0	58.1	4.2	0.0	0.124144	55018	55707	1520-1549	1.000000	80.5
rbcL_p1	rbcL	1	AGACCTTGTTGTTGCGAGAA	TGGGCCGAGTTTAATTGCAA	20	20	45.0	45.0	57.7	58.4	0.0	0.0	0.703591	59174	60655	1526-1580	1.000000	77.2
rbcL_p2	rbcL	2	AGACCTTGTTGTTGCGAGAA	TTGGGCCGAGTTTAATTGCA	20	20	45.0	45.0	57.7	58.4	0.0	0.0	0.703591	59174	60655	1527-1581	1.000000	77.2
rbcL_p3	rbcL	3	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	20	20	50.0	45.0	57.3	58.4	0.0	0.0	1.093771	59174	60655	1495-1549	1.000000	74.1
rbcL_p4	rbcL	4	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	20	20	50.0	45.0	57.3	58.4	0.0	0.0	1.093771	59174	60655	1496-1550	1.000000	74.1
rbcL_p5	rbcL	5	AGTAGACCTTGTTGTTGCGA	TGGGCCGAGTTTAATTGCAA	20	20	45.0	45.0	57.1	58.4	0.0	0.0	1.280713	59174	60655	1529-1583	1.000000	72.5
petA-psbJ_p1	petA-psbJ	1	CTACGTGTCCAAGGCCTTTT	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.1	57.6	17.6	0.0	0.527849	67540	68620	1221-1221	1.000000	75.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.574912	67540	68620	1209-1209	1.000000	75.5
petA-psbJ_p3	petA-psbJ	3	GATCCACTACGTGTCCAAGG	ATTCTGGATTGGGTTCGTCC	20	20	55.0	50.0	57.7	57.6	0.0	0.0	0.715311	67540	68620	1227-1227	1.000000	74.3
petA-psbJ_p4	petA-psbJ	4	TACGTGTCCAAGGCCTTTTG	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.4	57.6	17.6	0.0	0.820035	67540	68620	1220-1220	1.000000	73.5
petA-psbJ_p5	petA-psbJ	5	TGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	20	20	45.0	50.0	57.5	57.6	17.6	0.0	0.872659	67540	68620	1216-1216	1.000000	73.1
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.790741	72346	72719	487-547	1.000000	78.8
rpl33-rps18_p2	rpl33-rps18	2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	72346	72719	486-546	1.000000	76.3
rpl33-rps18_p3	rpl33-rps18	3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	72346	72719	488-548	1.000000	76.3
rpl33-rps18_p4	rpl33-rps18	4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.5	58.6	0.0	0.0	1.173970	72346	72719	484-544	1.000000	75.7
rpl33-rps18_p5	rpl33-rps18	5	ACGCCTAGTCGATTGGAATT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.236405	72346	72719	478-538	1.000000	75.2
clpP_p1	clpP	1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	22	20	40.9	45.0	57.8	57.7	0.0	3.0	2.422099	74651	76860	2276-2296	1.000000	62.3
clpP_p2	clpP	2	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	22	20	40.9	45.0	57.6	57.7	0.0	3.0	2.689259	74651	76860	2275-2295	1.000000	60.2
clpP_p3	clpP	3	GGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	21	20	42.9	45.0	56.1	57.7	0.0	3.0	3.124892	74651	76860	2275-2295	1.000000	56.7
clpP_p4	clpP	4	GGTGTTAAATCGTTGCTATTGCT	ACGCTTCCACATCAAAGTGA	23	20	39.1	45.0	58.0	57.7	0.0	3.0	3.272752	74651	76860	2270-2290	1.000000	55.5
clpP_p5	clpP	5	TGTGGATTGGTGTTAAATCGTTG	ACGCTTCCACATCAAAGTGA	23	20	39.1	45.0	57.9	57.7	0.0	3.0	3.369331	74651	76860	2278-2298	1.000000	54.7
petD_p1	petD	1	TTCCCCGAAGAGAAAATGGA	ACTCCCTAGATACGCACATCA	20	21	45.0	47.6	56.4	58.1	0.0	0.0	2.615939	81422	82679	1285-1324	1.000000	61.5
petD_p2	petD	2	TCCCCGAAGAGAAAATGGAT	ACTCCCTAGATACGCACATCA	20	21	45.0	47.6	56.2	58.1	0.0	0.0	2.851722	81422	82679	1284-1323	1.000000	59.6
petD_p3	petD	3	ATTCCCCGAAGAGAAAATGGA	ACTCCCTAGATACGCACATCA	21	21	42.9	47.6	57.0	58.1	0.0	0.0	3.075538	81422	82679	1286-1325	1.000000	57.8
petD_p4	petD	4	TCCCCGAAGAGAAAATGGATT	ACTCCCTAGATACGCACATCA	21	21	42.9	47.6	57.0	58.1	0.0	0.0	3.075538	81422	82679	1284-1323	1.000000	57.8
petD_p5	petD	5	TTCCCCGAAGAGAAAATGGAT	ACTCCCTAGATACGCACATCA	21	21	42.9	47.6	57.0	58.1	0.0	0.0	3.075538	81422	82679	1285-1324	1.000000	57.8
rpl32-ndhF_p1	rpl32-ndhF	1	AATGCCGCCCAATATCCTTT	GGGAAGTGGCACAAAAGGTA	20	20	45.0	50.0	57.9	58.0	0.0	21.4	0.096904	131696	132930	1364-1372	1.000000	84.9
rpl32-ndhF_p2	rpl32-ndhF	2	ATGCCGCCCAATATCCTTTT	GGGAAGTGGCACAAAAGGTA	20	20	45.0	50.0	57.9	58.0	0.0	21.4	0.096904	131696	132930	1363-1371	1.000000	84.9
rpl32-ndhF_p3	rpl32-ndhF	3	TGCCGCCCAATATCCTTTTT	GGGAAGTGGCACAAAAGGTA	20	20	45.0	50.0	58.1	58.0	0.0	21.4	0.150992	131696	132930	1362-1370	1.000000	84.5
rpl32-ndhF_p4	rpl32-ndhF	4	AATGCCGCCCAATATCCTTT	AAAGGAATGGGAAGTGGCAC	20	20	45.0	50.0	57.9	58.4	0.0	10.3	0.454022	131696	132930	1372-1380	1.000000	82.0
rpl32-ndhF_p5	rpl32-ndhF	5	ATGCCGCCCAATATCCTTTT	AAAGGAATGGGAAGTGGCAC	20	20	45.0	50.0	57.9	58.4	0.0	10.3	0.454022	131696	132930	1371-1379	1.000000	82.0