Hotspot

ycf1

SSC · 124506-130052 · 5547 bp

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Pi 0.00667

MarkerSeek score 56.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 37
pi: 0.00667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GATGCATCTCCCCTCTTTGA	AGATGTTCAAGAAAATTATTCGCA	50.0	29.2	57.0	55.7	242 bp	1.0	41.7
ycf1_p2	GATGCATCTCCCCTCTTTGA	TGTTCAAGAAAATTATTCGCAATCA	50.0	28.0	57.0	56.6	239 bp	1.0	41.7
ycf1_p3	AGATGCATCTCCCCTCTTTG	TGTTCAAGAAAATTATTCGCAATCA	50.0	28.0	57.0	56.6	240 bp	1.0	41.7
ycf1_p4	AGATGCATCTCCCCTCTTTG	AGATGTTCAAGAAAATTATTCGCA	50.0	29.2	57.0	55.7	243 bp	1.0	41.6
ycf1_p5	ACATCTAGATGCATCTCCCCT	AGATGTTCAAGAAAATTATTCGCA	47.6	29.2	57.7	55.7	249 bp	1.0	41.6