Hotspot

rbcL

LSC · 55028-56467 · 1440 bp

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Pi 0.00488

MarkerSeek score 61.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 7
pi: 0.00488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	GGGCCGAGTTTAATTGCAAC	50.0	50.0	57.3	58.0	1558-1563 bp	1.0	74.8
rbcL_p2	TGAGTTGTAGGGAGGGACTT	ATTAGTGAATTCCGCGTGCT	50.0	45.0	57.3	58.0	1513-1518 bp	1.0	74.7
rbcL_p3	TGAGTTGTAGGGAGGGACTT	CCGCGTGCTTGTTTATTCTC	50.0	50.0	57.3	57.8	1502-1507 bp	1.0	73.6
rbcL_p4	TGAGTTGTAGGGAGGGACTT	TCCGCGTGCTTGTTTATTCT	50.0	45.0	57.3	58.2	1503-1508 bp	1.0	73.3
rbcL_p5	TGAGTTGTAGGGAGGGACTT	TTAGTGAATTCCGCGTGCTT	50.0	45.0	57.3	58.2	1512-1517 bp	1.0	73.3