Hotspot

atpF

LSC · 11973-13330 · 1358 bp

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Pi 0.00884

MarkerSeek score 49.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1358
variable_sites: 12
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAATAAGCCTCGCTCACAGG	GACTTGCCGTTTGCTTCTTC	50.0	50.0	58.0	58.0	2114-2116 bp	1.0	80.1
atpF_p2	AAATAAGCCTCGCTCACAGG	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2408-2410 bp	1.0	80.1
atpF_p3	CACCCGCCATTACTTCATCA	GACTTGCCGTTTGCTTCTTC	50.0	50.0	58.0	58.0	1954-1956 bp	1.0	80.0
atpF_p4	AAATAAGCCTCGCTCACAGG	GCAGAGGGGAAAATACGAGG	50.0	55.0	58.0	58.0	2288-2290 bp	1.0	80.0
atpF_p5	AAATAAGCCTCGCTCACAGG	GGCAGAGGGGAAAATACGAG	50.0	55.0	58.0	58.0	2289-2291 bp	1.0	80.0