Hotspot

rps15-ycf1

SSC · 124075-124505 · 431 bp

Back to Bomarea

Pi 0.0209
MarkerSeek score 67.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
431
variable_sites
9
pi
0.0209
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGACCCCTTTGTTTCT GCCGTTTCAGCATGTTAAGG 42.9 50.0 57.3 57.7 552-555 bp 1.0 65.9
rps15-ycf1_p2 ACAGACCCCTTTGTTTCTTCT GCCGTTTCAGCATGTTAAGG 42.9 50.0 57.0 57.7 549-552 bp 1.0 63.6
rps15-ycf1_p3 TCAACAGACCCCTTTGTTTCTT GCCGTTTCAGCATGTTAAGG 40.9 50.0 58.0 57.7 552-555 bp 1.0 63.0
rps15-ycf1_p4 TTCAACAGACCCCTTTGTTTCT GCCGTTTCAGCATGTTAAGG 40.9 50.0 58.0 57.7 553-556 bp 1.0 63.0
rps15-ycf1_p5 CAAATGGGAAGTAAGTCTCCGT GCCGTTTCAGCATGTTAAGG 45.5 50.0 58.1 57.7 604-607 bp 1.0 62.6