Hotspot

ccsA-ndhD

SSC · 115637-115917 · 281 bp

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Pi 0.0463

MarkerSeek score 79.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 281
variable_sites: 13
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGAATTCAGCACTTGTGGCT	TCGGGATCGGTATTTATCCTG	45.0	47.6	58.0	56.8	474 bp	1.0	64.2
ccsA-ndhD_p2	ATTCAGCACTTGTGGCTTCT	TCGGGATCGGTATTTATCCTG	45.0	47.6	57.7	56.8	471 bp	1.0	62.1
ccsA-ndhD_p3	CTGAATTCAGCACTTGTGGC	TCGGGATCGGTATTTATCCTG	50.0	47.6	57.7	56.8	475 bp	1.0	61.6
ccsA-ndhD_p4	TGAATTCAGCACTTGTGGCT	ATCGGGATCGGTATTTATCCTG	45.0	45.5	58.0	57.3	475 bp	1.0	60.2
ccsA-ndhD_p5	TGAATTCAGCACTTGTGGCT	CGGGATCGGTATTTATCCTG	45.0	50.0	58.0	55.2	473 bp	1.0	59.7