Hotspot

rpl32-trnL-UAG

SSC · 113727-114514 · 788 bp

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Pi 0.0102
MarkerSeek score 64.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
788
variable_sites
8
pi
0.0102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACTGCAGAAAAGGCTCTTTCT AGCGTGTCTACCGATTTCAC 42.9 50.0 57.8 58.0 913-914 bp 1.0 70.9
rpl32-trnL-UAG_p2 ACTGCAGAAAAGGCTCTTTCT CAGCGTGTCTACCGATTTCA 42.9 50.0 57.8 58.0 914-915 bp 1.0 70.9
rpl32-trnL-UAG_p3 ACTGCAGAAAAGGCTCTTTCT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.8 57.9 923-924 bp 1.0 69.9
rpl32-trnL-UAG_p4 ACTGCAGAAAAGGCTCTTTCT CCTAAGAGCAGCGTGTCTAC 42.9 55.0 57.8 57.8 922-923 bp 1.0 69.4
rpl32-trnL-UAG_p5 ACTGCAGAAAAGGCTCTTTCT CTAAGAGCAGCGTGTCTACC 42.9 55.0 57.8 57.8 921-922 bp 1.0 69.4