Hotspot

atpB-rbcL

LSC · 54237-55027 · 791 bp

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Pi 0.00632

MarkerSeek score 62.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 5
pi: 0.00632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GCTTTGAATCCAGCACTTGC	50.0	50.0	58.1	58.3	944 bp	1.0	77.3
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GCTTTGAATCCAGCACTTGC	45.0	50.0	57.9	58.3	922 bp	1.0	77.0
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	GGCTTTGAATCCAGCACTTG	50.0	50.0	58.1	57.6	945 bp	1.0	76.8
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	GGCTTTGAATCCAGCACTTG	45.0	50.0	57.9	57.6	923 bp	1.0	76.5
atpB-rbcL_p5	GGGGAAAAACGACATCCAGT	TAACACCGGCTTTGAATCCA	50.0	45.0	58.1	57.4	952 bp	1.0	75.1