Hotspot

trnT-GGU-psbD

LSC · 31668-32667 · 1000 bp

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Pi 0.00405

MarkerSeek score 62.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 4
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1151-1164 bp	1.0	80.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1154-1167 bp	1.0	80.4
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1153-1166 bp	1.0	80.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1156-1169 bp	1.0	80.2
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1128-1141 bp	1.0	78.9