Hotspot

matK-trnK-UUU

LSC · 3230-4037 · 808 bp

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Pi 0.00867

MarkerSeek score 61.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 7
pi: 0.00867
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCATGAGCAAGCGTGTAAA	TCGGGTTGCTAACTCAATGG	45.0	50.0	58.1	57.9	949 bp	1.0	78.9
matK-trnK-UUU_p2	ACCATGAGCAAGCGTGTAAA	CGGGTTGCTAACTCAATGGT	45.0	50.0	58.1	58.2	948 bp	1.0	78.4
matK-trnK-UUU_p3	ACTCCTGAAAGAGAAGCGGA	TCGGGTTGCTAACTCAATGG	50.0	50.0	58.4	57.9	926 bp	1.0	76.9
matK-trnK-UUU_p4	ACTCCTGAAAGAGAAGCGGA	CGGGTTGCTAACTCAATGGT	50.0	50.0	58.4	58.2	925 bp	1.0	76.3
matK-trnK-UUU_p5	GACCATGAGCAAGCGTGTAA	TCGGGTTGCTAACTCAATGG	50.0	50.0	58.6	57.9	950 bp	1.0	75.3