Hotspot

ycf1

SSC · 120901-124902 · 4002 bp

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Pi 0.0493

MarkerSeek score 69.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4002
variable_sites: 600
pi: 0.0493
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTCCGCGCAGTTCAAAATT	TATCCGTTTCAGCTGAAGCC	45.0	50.0	57.9	58.0	1315-1342 bp	1.0	87.4
ycf1_p2	CATCTGTGAGGTGCTACGTC	TATCCGTTTCAGCTGAAGCC	55.0	50.0	58.1	58.0	1436-1463 bp	0.888889	82.7
ycf1_p3	CTTCCGCGCAGTTCAAAATT	ATTGCGTATCCGTTTCAGCT	45.0	45.0	57.9	58.0	1321-1348 bp	0.666667	74.1
ycf1_p4	CATCTGTGAGGTGCTACGTC	ATTGCGTATCCGTTTCAGCT	55.0	45.0	58.1	58.0	1442-1469 bp	0.555556	69.3
ycf1_p5	CTTCCGCGCAGTTCAAAATT	TTGGCCGTATATTGTTCCGG	45.0	50.0	57.9	58.0	1473-1500 bp	0.333333	56.7