Hotspot

ycf1-chlN

SSC · 124903-126381 · 1479 bp

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Pi 0.0619

MarkerSeek score 80.20

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 1479
variable_sites: 256
pi: 0.0619
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	GTTTTCCCTTCGCGAAAGTG	AGTTACTCGTCCATTGCGAC	50.0	50.0	58.0	58.0	2258-2307 bp	0.777778	79.4
ycf1-chlN_p2	GGTGTCTCCGACCATTTACC	CAGTTCCTGTATCGGGTTGG	55.0	55.0	58.0	58.0	2739-2785 bp	0.777778	79.3
ycf1-chlN_p3	GCCCCCTTTCCCCTTTTTAA	AGTTACTCGTCCATTGCGAC	50.0	50.0	58.0	58.0	1741-1790 bp	0.666667	74.9
ycf1-chlN_p4	GGTGTCTCCGACCATTTACC	AGTTACTCGTCCATTGCGAC	55.0	50.0	58.0	58.0	2087-2133 bp	0.555556	70.6
ycf1-chlN_p5	GGGTGTCTCCGACCATTTAC	AGTTACTCGTCCATTGCGAC	55.0	50.0	58.0	58.0	2094 bp	0.111111	44.2