Hotspot

ndhE-ndhG

SSC · 115413-115573 · 161 bp

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Pi 0.0569

MarkerSeek score 79.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 161
variable_sites: 25
pi: 0.0569
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCTAGGGGCAGAGGCTATC	GTCGTGTCTGCTGTAATGGT	55.0	50.0	58.0	57.9	1145-1196 bp	0.555556	66.9
ndhE-ndhG_p2	TTCTAGGGGCAGAGGCTATC	CATAGGGCACTTTACCGCTT	55.0	50.0	58.0	58.0	1086-1142 bp	0.444444	61.4
ndhE-ndhG_p3	CACACTGAGTGCAACCGATA	CATAGGGCACTTTACCGCTT	50.0	50.0	57.9	58.0	1005-1061 bp	0.444444	61.1
ndhE-ndhG_p4	CACTGAGTGCAACCGATACA	CATAGGGCACTTTACCGCTT	50.0	50.0	57.9	58.0	1003-1059 bp	0.444444	61.1
ndhE-ndhG_p5	TTCTAGGGGCAGAGGCTATC	GAGACTCTTCTGGCAGATGC	55.0	55.0	58.0	58.1	980-1031 bp	0.222222	52.1