Hotspot

rpl32-trnP-GGG

SSC · 110590-110971 · 382 bp

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Pi 0.0909
MarkerSeek score 75.50
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
382
variable_sites
97
pi
0.0909
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 GACCTAGTTGCGGGAAGAAG CATGTCGTCGGGTCATTCTT 55.0 50.0 58.0 58.0 1091 bp 0.111111 44.3
rpl32-trnP-GGG_p2 TCTTTGGCCCAATCTGCTTT CATGTCGTCGGGTCATTCTT 45.0 50.0 58.0 58.0 965 bp 0.111111 44.3
rpl32-trnP-GGG_p3 TTCTTTGGCCCAATCTGCTT CATGTCGTCGGGTCATTCTT 45.0 50.0 58.0 58.0 966 bp 0.111111 44.3
rpl32-trnP-GGG_p4 TTTCTTTGGCCCAATCTGCT CATGTCGTCGGGTCATTCTT 45.0 50.0 58.0 58.0 967 bp 0.111111 44.3
rpl32-trnP-GGG_p5 GACCTAGTTGCGGGAAGAAG ATAGAGGTTAGGATGCCGCT 55.0 50.0 58.0 58.0 1038 bp 0.111111 44.2