Hotspot

rpl14-rpl16

LSC · 78026-78306 · 281 bp

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Pi 0.0688

MarkerSeek score 77.30

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 57
pi: 0.0688
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCTACCACTGCTCTAACCAC	GTCAATAACGCGCTATGCAC	55.0	50.0	58.0	58.0	671-705 bp	0.777778	79.6
rpl14-rpl16_p2	ACTGGGCCAAAGATCCTAGT	GTCAATAACGCGCTATGCAC	50.0	50.0	58.0	58.0	788-822 bp	0.777778	79.3
rpl14-rpl16_p3	ACTGGGCCAAAGATCCTAGT	ACACAAAATGCCTGTGCCTA	50.0	45.0	58.0	58.0	578-612 bp	0.666667	75.7
rpl14-rpl16_p4	GCTACCACTGCTCTAACCAC	AGGGATCTCCGGAATACTGG	55.0	55.0	58.0	58.0	567-601 bp	0.666667	75.1
rpl14-rpl16_p5	GCTACCACTGCTCTAACCAC	ACACAAAATGCCTGTGCCTA	55.0	45.0	58.0	58.0	461-495 bp	0.555556	70.9