Hotspot

rpl20-rps12

LSC · 65598-66511 · 914 bp

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Pi 0.0494

MarkerSeek score 82.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 135
pi: 0.0494
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACTTCGTCGATTCGTCAGTG	GGAAGTGTAGCCGTATGCAA	50.0	50.0	58.0	58.0	2272-2311 bp	1.0	89.9
rpl20-rps12_p2	CAGTGAGCAGGGAAGAATCC	GGAAGTGTAGCCGTATGCAA	55.0	50.0	58.0	58.0	2257-2296 bp	1.0	89.9
rpl20-rps12_p3	AGTGAGCAGGGAAGAATCCT	GGAAGTGTAGCCGTATGCAA	50.0	50.0	58.0	58.0	2256-2295 bp	1.0	89.9
rpl20-rps12_p4	ACTTCGTCGATTCGTCAGTG	TTGCTTGTACGGTTGAGCTT	50.0	45.0	58.0	58.0	2246-2285 bp	1.0	89.8
rpl20-rps12_p5	ACTTCGTCGATTCGTCAGTG	TTTGCTTGTACGGTTGAGCT	50.0	45.0	58.0	58.0	2247-2286 bp	1.0	89.8