Hotspot

trnW-CCA-trnP-UGG

LSC · 63556-63738 · 183 bp

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Pi 0.0741

MarkerSeek score 79.20

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 36
pi: 0.0741
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	ACCTACGACATCGGGTTTTG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.9	57.9	290-299 bp	1.0	89.0
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	CGGTAGCGTGTTTGTTTTGG	50.0	50.0	57.9	58.3	291-300 bp	1.0	88.0
trnW-CCA-trnP-UGG_p3	CTACGACATCGGGTTTTGGA	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	288-297 bp	1.0	86.7
trnW-CCA-trnP-UGG_p4	TACGACATCGGGTTTTGGAG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	287-296 bp	1.0	86.7
trnW-CCA-trnP-UGG_p5	TTTGAACCTACGACATCGGG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.6	57.9	295-304 bp	1.0	86.6