Hotspot

cemA-psbJ

LSC · 59319-61303 · 1985 bp

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Pi 0.042

MarkerSeek score 80.40

Primer available yes

Label cemA-psbJ

Feature Metadata

label_name: cemA-psbJ
feature_type: igs
region: LSC
length_bp: 1985
variable_sites: 241
pi: 0.042
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-psbJ_p1	CCGTCAATTGTAGCAACTTATCA	TGGGGTCGTCTCTTCGATAA	39.1	50.0	57.2	57.9	1984-2044 bp	0.777778	51.2
cemA-psbJ_p2	GCAACTTATCATACAATGAGTGGG	TGGGGTCGTCTCTTCGATAA	41.7	50.0	57.9	57.9	1972-2032 bp	0.777778	49.1
cemA-psbJ_p3	ATCATACAATGAGTGGGTGA	TGGGGTCGTCTCTTCGATAA	40.0	50.0	53.7	57.9	1965-2025 bp	0.777778	47.3
cemA-psbJ_p4	CCGTCAATTGTAGCAACTTATCA	TTGGGGTCGTCTCTTCGATA	39.1	50.0	57.2	57.9	1985-2045 bp	0.444444	35.1
cemA-psbJ_p5	GCAACTTATCATACAATGAGTGGG	TTGGGGTCGTCTCTTCGATA	41.7	50.0	57.9	57.9	1973-2033 bp	0.444444	33.0