Hotspot

trnF-GAA-ndhJ

LSC · 47341-47566 · 226 bp

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Pi 0.0644

MarkerSeek score 77.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 226
variable_sites: 40
pi: 0.0644
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	ACTGAAAATCCCCGTGTCAC	TGTCGTACCCAACTTCTATGA	50.0	42.9	58.1	56.1	305-334 bp	1.0	68.1
trnF-GAA-ndhJ_p2	GACTGAAAATCCCCGTGTCA	TGTCGTACCCAACTTCTATGA	50.0	42.9	57.8	56.1	306-335 bp	1.0	67.5
trnF-GAA-ndhJ_p3	ACTGAAAATCCCCGTGTCAC	TGTCGTACCCAACTTCTATGAA	50.0	40.9	58.1	56.9	305-334 bp	1.0	66.0
trnF-GAA-ndhJ_p4	CCCGTGTCACCAGTTCAATT	TGTCGTACCCAACTTCTATGA	50.0	42.9	58.4	56.1	295-324 bp	1.0	65.8
trnF-GAA-ndhJ_p5	GACTGAAAATCCCCGTGTCA	TGTCGTACCCAACTTCTATGAA	50.0	40.9	57.8	56.9	306-335 bp	1.0	65.4