Hotspot

rps4-trnL-UAA

LSC · 45571-46248 · 678 bp

Back to Bolbitis

Pi 0.0568

MarkerSeek score 79.10

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 113
pi: 0.0568
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GACGCACGCAAAATTGAGAA	GCCTGATAAATGGTCCCAGG	45.0	55.0	58.0	58.0	923-975 bp	1.0	89.9
rps4-trnL-UAA_p2	GACGCACGCAAAATTGAGAA	GGGGGCTGTCACTTGATATG	45.0	55.0	58.0	58.0	890-942 bp	1.0	89.9
rps4-trnL-UAA_p3	GACGCACGCAAAATTGAGAA	CTCCTTGGGGATAGAGGGAG	45.0	60.0	58.0	58.0	1515-1585 bp	1.0	89.7
rps4-trnL-UAA_p4	CGGGAATTGTGGAAGCCATA	GCCTGATAAATGGTCCCAGG	50.0	55.0	57.9	58.0	1097-1149 bp	1.0	89.4
rps4-trnL-UAA_p5	CGGGAATTGTGGAAGCCATA	CTCCTTGGGGATAGAGGGAG	50.0	60.0	57.9	58.0	1689-1759 bp	1.0	89.3