Hotspot

psbD-trnT-GGU

LSC · 34835-36208 · 1374 bp

Back to Bolbitis

Pi 0.054

MarkerSeek score 79.70

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1374
variable_sites: 213
pi: 0.054
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AACCCACGAAGACAAAACGA	GCAAAAACTAGTCGTGTGCC	45.0	50.0	58.0	58.0	1584-1604 bp	0.555556	71.0
psbD-trnT-GGU_p2	CAAGGAGTGAGCCAAACTGT	GCAAAAACTAGTCGTGTGCC	50.0	50.0	58.0	58.0	1757-1777 bp	0.555556	70.3
psbD-trnT-GGU_p3	CGCAACCCCCATCATATGAA	GCAAAAACTAGTCGTGTGCC	50.0	50.0	58.0	58.0	2096-2116 bp	0.555556	69.7
psbD-trnT-GGU_p4	AGCGACGAAGGTCCATAAAC	GCAAAAACTAGTCGTGTGCC	50.0	50.0	58.0	58.0	1835-1855 bp	0.333333	60.0
psbD-trnT-GGU_p5	AGCGACGAAGGTCCATAAAC	ACGGTGCAAAAACTAGTCGT	50.0	45.0	58.0	58.1	1860 bp	0.111111	43.9