Hotspot

trnE-UUC-psbM

LSC · 27438-27902 · 465 bp

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Pi 0.0439

MarkerSeek score 77.40

Primer available yes

Label trnE-UUC-psbM

Feature Metadata

label_name: trnE-UUC-psbM
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 63
pi: 0.0439
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbM_p1	ATCCCTCCCATCGATAGCAA	TTGAATCATCCCCTGCGAAG	50.0	50.0	58.0	58.0	1478 bp	0.111111	43.9
trnE-UUC-psbM_p2	TCCCTCCCATCGATAGCAAT	TTGAATCATCCCCTGCGAAG	50.0	50.0	58.0	58.0	1477 bp	0.111111	43.9
trnE-UUC-psbM_p3	TCTATCCTCGGACTTGGGTC	TTGAATCATCCCCTGCGAAG	55.0	50.0	57.9	58.0	1212 bp	0.111111	43.6
trnE-UUC-psbM_p4	CTCATCGTTGACTCTGAGGC	TTGAATCATCCCCTGCGAAG	55.0	50.0	58.1	58.0	1378 bp	0.111111	43.5
trnE-UUC-psbM_p5	CATCGTTGACTCTGAGGCAA	TTGAATCATCCCCTGCGAAG	50.0	50.0	57.9	58.0	1376 bp	0.111111	43.4