Hotspot

ndhA

SSC · 127719-129877 · 2159 bp

Back to Boesenbergia

Pi 0.0139

MarkerSeek score 64.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2159
variable_sites: 30
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTCACCATTGGAAAC	GTCCACCCGGCTTCATAAAT	50.0	50.0	58.0	57.9	2394-2401 bp	1.0	80.6
ndhA_p2	CCCAGTCACCATTGGAAACA	GTCCACCCGGCTTCATAAAT	50.0	50.0	58.0	57.9	2393-2400 bp	1.0	80.6
ndhA_p3	ATGAACCCAGTCACCATTGG	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.8	57.9	2398-2405 bp	1.0	78.9
ndhA_p4	GAACCCAGTCACCATTGGAA	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.7	57.9	2396-2403 bp	1.0	78.4
ndhA_p5	AACCCAGTCACCATTGGAAA	GTCCACCCGGCTTCATAAAT	45.0	50.0	57.2	57.9	2395-2402 bp	1.0	74.6