Hotspot

rpl2-trnH-GUG

IRa · 162518-162563 · 46 bp

Back to Boesenbergia

Pi 0.0652

MarkerSeek score 70.20

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 46
variable_sites: 3
pi: 0.0652
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	GGGTTCAATTCCCGTCGTT	50.0	52.6	58.0	58.1	146-149 bp	1.0	72.9
rpl2-trnH-GUG_p2	CACAACCCCTTGGGGTTATC	GGGTTCAATTCCCGTCGTT	55.0	52.6	58.2	58.1	160-163 bp	1.0	71.8
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GGGTTCAATTCCCGTCGTTC	50.0	55.0	58.0	59.2	146-149 bp	1.0	71.8
rpl2-trnH-GUG_p4	CACAACCCCTTGGGGTTATC	GGGTTCAATTCCCGTCGTTC	55.0	55.0	58.2	59.2	160-163 bp	1.0	70.7
rpl2-trnH-GUG_p5	TATCCTGCGCTTGGAAGAAG	GGGTTCAATTCCCGTCGTT	50.0	52.6	57.7	58.1	144-147 bp	1.0	70.6