Hotspot

ndhF-rpl32

SSC · 120012-120662 · 651 bp

Back to Boesenbergia

Pi 0.042
MarkerSeek score 76.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
651
variable_sites
27
pi
0.042
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGAATAAGTAGAAGCCCGA AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 840-896 bp 1.0 60.7
ndhF-rpl32_p2 CGGAATAAGTAGAAGCCCGA GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 839-895 bp 1.0 60.7
ndhF-rpl32_p3 TCGGAATAAGTAGAAGCCCG AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 841-897 bp 1.0 60.7
ndhF-rpl32_p4 TCGGAATAAGTAGAAGCCCG GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 840-896 bp 1.0 60.7
ndhF-rpl32_p5 GAAGCCCGAATCCTATTGACA GAAAAAGCCTTTACTGCAGCT 47.6 42.9 57.8 57.3 828-884 bp 1.0 60.5