Hotspot

rrn16-trnI-GAU

IRb · 106795-107099 · 305 bp

Back to Boesenbergia

Pi 0.0328
MarkerSeek score 65.30
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
IRb
length_bp
305
variable_sites
10
pi
0.0328
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 CAAGGTAGCCGTACTGGAAG CTATCCATGTGGCTGAGAGC 55.0 55.0 57.7 57.8 431 bp 1.0 78.2
rrn16-trnI-GAU_p2 CAAGGTAGCCGTACTGGAAG CGCTGATGAGCACATTGAAC 55.0 50.0 57.7 57.8 450 bp 1.0 78.1
rrn16-trnI-GAU_p3 CAAGGTAGCCGTACTGGAAG GGCACAACGACGCAATTATC 55.0 50.0 57.7 58.2 399 bp 1.0 78.1
rrn16-trnI-GAU_p4 AGTGACTGGAGTGAAGTCGT CTATCCATGTGGCTGAGAGC 50.0 55.0 58.3 57.8 453 bp 1.0 78.0
rrn16-trnI-GAU_p5 AGTGACTGGAGTGAAGTCGT CGCTGATGAGCACATTGAAC 50.0 50.0 58.3 57.8 472 bp 1.0 77.9