Hotspot

trnH-GUG-rpl2

IRb · 88645-88690 · 46 bp

Back to Boesenbergia

Pi 0.0652

MarkerSeek score 70.20

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 46
variable_sites: 3
pi: 0.0652
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	GGGTTCAATTCCCGTCGTT	GTTATCCTGCGCTTGGAAGA	52.6	50.0	58.1	58.0	146-149 bp	1.0	72.9
trnH-GUG-rpl2_p2	GGGTTCAATTCCCGTCGTT	CACAACCCCTTGGGGTTATC	52.6	55.0	58.1	58.2	160-163 bp	1.0	71.8
trnH-GUG-rpl2_p3	GGGTTCAATTCCCGTCGTTC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	59.2	58.0	146-149 bp	1.0	71.8
trnH-GUG-rpl2_p4	GGGTTCAATTCCCGTCGTTC	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	160-163 bp	1.0	70.7
trnH-GUG-rpl2_p5	GGGTTCAATTCCCGTCGTT	TATCCTGCGCTTGGAAGAAG	52.6	50.0	58.1	57.7	144-147 bp	1.0	70.6