Hotspot

petD-rpoA

LSC · 81708-81894 · 187 bp

Back to Boesenbergia

Pi 0.0214

MarkerSeek score 64.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 4
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CGTCGTCCAGTAGCTACAAC	TCAGACACTTTACGGAAGCA	55.0	45.0	58.1	56.8	373 bp	1.0	70.5
petD-rpoA_p2	TTTTTGATCGGTACCGCAGT	TCAGACACTTTACGGAAGCA	45.0	45.0	57.8	56.8	349 bp	1.0	70.0
petD-rpoA_p3	TCGTCGTCCAGTAGCTACAA	TCAGACACTTTACGGAAGCA	50.0	45.0	58.2	56.8	374 bp	1.0	69.8
petD-rpoA_p4	TTCGTCGTCCAGTAGCTACA	TCAGACACTTTACGGAAGCA	50.0	45.0	58.2	56.8	375 bp	1.0	69.8
petD-rpoA_p5	CATTTCGTCGTCCAGTAGCT	TCAGACACTTTACGGAAGCA	50.0	45.0	57.7	56.8	378 bp	1.0	69.1