Hotspot

atpB-rbcL

LSC · 58137-58971 · 835 bp

Back to Boesenbergia

Pi 0.0085

MarkerSeek score 64.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 7
pi: 0.0085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCCTAGGTTTTGTGTGGAA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.0	56.6	911-926 bp	1.0	70.5
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	934-949 bp	1.0	69.5
atpB-rbcL_p3	TACGCCCTAGGTTTTGTGTG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.8	56.6	914-929 bp	1.0	69.2
atpB-rbcL_p4	CGATACGCCCTAGGTTTTGT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.7	56.6	917-932 bp	1.0	68.1
atpB-rbcL_p5	GTTTTGTGTGGAAACCGCAG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.4	56.6	904-919 bp	1.0	67.1