Hotspot

ycf3-trnS-GGA

LSC · 47075-47707 · 633 bp

Back to Boesenbergia

Pi 0.0243

MarkerSeek score 67.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 15
pi: 0.0243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCCACAATTGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	779-787 bp	1.0	81.2
ycf3-trnS-GGA_p2	TGGCCACAATTGAAGAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	729-737 bp	1.0	81.1
ycf3-trnS-GGA_p3	TTTTCTCCGGAAGTTGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	819-827 bp	1.0	80.8
ycf3-trnS-GGA_p4	TTTTCTCCGGAAGTTGTCGG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	769-777 bp	1.0	80.7
ycf3-trnS-GGA_p5	GGCCACAATTGAAGAGGTCT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	778-786 bp	1.0	80.3