Hotspot

psbC-trnS-UGA

LSC · 38004-38149 · 146 bp

Back to Boesenbergia

Pi 0.0548
MarkerSeek score 81.80
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
146
variable_sites
8
pi
0.0548
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CTTTCCATGACCCCTCTTCG GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.0 58.0 261-264 bp 1.0 82.0
psbC-trnS-UGA_p2 CATTTGTGGCATGCAGGAAG GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.0 58.0 339-342 bp 1.0 81.7
psbC-trnS-UGA_p3 CATTTGTGGCATGCAGGAAG GCTGAGTGGTTGATAGCTCC 50.0 55.0 58.0 58.1 331-334 bp 1.0 81.5
psbC-trnS-UGA_p4 CATTTGTGGCATGCAGGAAG GGCTGAGTGGTTGATAGCTC 50.0 55.0 58.0 58.1 332-335 bp 1.0 81.5
psbC-trnS-UGA_p5 CATTTGTGGCATGCAGGAAG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.0 58.1 324-327 bp 1.0 81.5