Hotspot

atpF-atpH

LSC · 13483-13978 · 496 bp

Back to Boesenbergia

Pi 0.0283

MarkerSeek score 59.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 14
pi: 0.0283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTCGTCGATTCGGCATTAGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.9	58.0	1098-1112 bp	1.0	81.3
atpF-atpH_p2	GTCGTCGATTCGGCATTAGA	TATTGCGAGACAACCGGAAG	50.0	50.0	57.9	58.0	1187-1201 bp	1.0	81.2
atpF-atpH_p3	GTCGTCGATTCGGCATTAGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	57.9	58.0	1288-1302 bp	1.0	80.8
atpF-atpH_p4	TCGTCGAGATTGTGCACTTT	TATTGCGAGACAACCGGAAG	45.0	50.0	57.9	58.0	876-899 bp	1.0	80.6
atpF-atpH_p5	TTCGTCGAGATTGTGCACTT	TATTGCGAGACAACCGGAAG	45.0	50.0	57.9	58.0	877-900 bp	1.0	80.6