Hotspot

rps15

SSC · 123596-123868 · 273 bp

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Pi 0.466
MarkerSeek score 76.50
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
117
pi
0.466
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TCCCAACGCGTTACATAAGG GCTCGCGAAAGAAATCAACA 50.0 45.0 58.0 57.4 832 bp 0.5 55.1
rps15_p2 TTGATATTCAGCAACCCCGG GCTCGCGAAAGAAATCAACA 50.0 45.0 57.9 57.4 1190 bp 0.5 54.8
rps15_p3 GATATTCAGCAACCCCGGTT GCTCGCGAAAGAAATCAACA 50.0 45.0 57.9 57.4 1188 bp 0.5 54.8
rps15_p4 AACACCATGCATTGATGGGT GCTCGCGAAAGAAATCAACA 45.0 45.0 58.1 57.4 695 bp 0.5 54.8
rps15_p5 TCAGCAACCCCGGTTAAAAA GCTCGCGAAAGAAATCAACA 45.0 45.0 57.9 57.4 1183 bp 0.5 54.7