Hotspot

ndhI

SSC · 119612-120103 · 492 bp

Pi 0.507

MarkerSeek score 77.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 492
variable_sites: 205
pi: 0.507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CCCAACGGTCCAAAGAGAAA	CTCTAGCCGAGTGTGAGAGA	50.0	55.0	58.0	58.0	1643 bp	0.5	59.6
ndhI_p2	CCCAACGGTCCAAAGAGAAA	TCTCTAGCCGAGTGTGAGAG	50.0	55.0	58.0	58.0	1644 bp	0.5	59.6
ndhI_p3	GCCAAGTAACCGTTCGTTTG	CTCTAGCCGAGTGTGAGAGA	50.0	55.0	58.0	58.0	1111 bp	0.5	59.5
ndhI_p4	GCCAAGTAACCGTTCGTTTG	TCTCTAGCCGAGTGTGAGAG	50.0	55.0	58.0	58.0	1112 bp	0.5	59.5
ndhI_p5	TGATCCCATACCACGACGTA	CTCTAGCCGAGTGTGAGAGA	50.0	55.0	57.9	58.0	1725 bp	0.5	59.3