Hotspot

ndhH

SSC · 122317-123498 · 1182 bp

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Pi 0.503
MarkerSeek score 82.80
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
547
pi
0.503
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 AGCAGCCCTTAATCCACCTA GCTCGCGAAAGAAATCAACA 50.0 45.0 58.1 57.4 2298 bp 0.5 54.4
ndhH_p2 AGCAGCCCTTAATCCACCTA CATCTCAGAGAGGTTTGCGA 50.0 50.0 58.1 57.3 1906 bp 0.5 53.9
ndhH_p3 AGCAGCCCTTAATCCACCTA TCATCTCAGAGAGGTTTGCG 50.0 50.0 58.1 57.3 1907 bp 0.5 53.9
ndhH_p4 GATTGAGCAGCAGCCCTTAA GCTCGCGAAAGAAATCAACA 50.0 45.0 58.2 57.4 2306 bp 0.5 53.3
ndhH_p5 TTGAGCAGCAGCCCTTAATC GCTCGCGAAAGAAATCAACA 50.0 45.0 58.2 57.4 2304 bp 0.5 53.3