Hotspot

ndhG

SSC · 118719-119249 · 531 bp

Pi 0.467

MarkerSeek score 79.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 213
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGATTTAGGAGCCAGGACCA	CAAACGAACGGTTACTTGGC	50.0	50.0	58.0	58.0	1440 bp	0.5	59.5
ndhG_p2	TGATTTAGGAGCCAGGACCA	CCACATCACCCCCATTCAAA	50.0	50.0	58.0	58.1	1299 bp	0.5	59.2
ndhG_p3	TGATTTAGGAGCCAGGACCA	ACATCAGAACGTTTCCGAGG	50.0	50.0	58.0	57.9	1886 bp	0.5	59.1
ndhG_p4	TGATTTAGGAGCCAGGACCA	AACGGTTACTTGGCGCTAAT	50.0	45.0	58.0	57.9	1434 bp	0.5	58.9
ndhG_p5	TGATTTAGGAGCCAGGACCA	ACGGTTACTTGGCGCTAATT	50.0	45.0	58.0	57.9	1433 bp	0.5	58.9