Hotspot

ndhF

SSC · 110897-113143 · 2247 bp

Pi 0.452

MarkerSeek score 80.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 906
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	TCGGGAAAATACGCCCAATT	50.0	45.0	58.0	57.9	2540 bp	0.5	58.6
ndhF_p2	GCAGAATACCGTCACCCATT	CTCGGGAAAATACGCCCAAT	50.0	50.0	58.0	58.3	2541 bp	0.5	57.1
ndhF_p3	GCAGAATACCGTCACCCATT	CAAACTCGGGAAAATACGCC	50.0	50.0	58.0	57.5	2545 bp	0.5	55.4
ndhF_p4	GCAGAATACCGTCACCCATT	AAACTCGGGAAAATACGCCC	50.0	50.0	58.0	58.5	2544 bp	0.5	55.3
ndhF_p5	GCAGAATACCGTCACCCATT	TGAGAACCCAACTTTCCGTT	50.0	45.0	58.0	57.3	2603 bp	0.5	53.9