Hotspot

ndhE

SSC · 118215-118520 · 306 bp

Pi 0.491

MarkerSeek score 77.10

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 141
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGATTTAGGAGCCAGGACCA	TTTCTCTTTGGACCGTTGGG	50.0	50.0	58.0	58.0	908 bp	0.5	59.5
ndhE_p2	AACCAACACAGTCTTCGGTC	TTTCTCTTTGGACCGTTGGG	50.0	50.0	58.1	58.0	1178 bp	0.5	59.4
ndhE_p3	TGATTTAGGAGCCAGGACCA	TACGTCGTGGTATGGGATCA	50.0	50.0	58.0	57.9	826 bp	0.5	59.3
ndhE_p4	ACACTCAAGAAATCGGTCGG	TTTCTCTTTGGACCGTTGGG	50.0	50.0	57.9	58.0	1221 bp	0.5	59.2
ndhE_p5	AACCAACACAGTCTTCGGTC	TACGTCGTGGTATGGGATCA	50.0	50.0	58.1	57.9	1096 bp	0.5	59.2