Hotspot

ndhD

SSC · 116092-117621 · 1530 bp

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Pi 0.483
MarkerSeek score 76.60
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1530
variable_sites
520
pi
0.483
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.70
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TTTGTGTGAACAGGCGAGAA TGGTCCTGGCTCCTAAATCA 45.0 50.0 58.0 58.0 2176 bp 0.5 59.5
ndhD_p2 TTTGTGTGAACAGGCGAGAA GACCGAAGACTGTGTTGGTT 45.0 50.0 58.0 58.1 1906 bp 0.5 59.3
ndhD_p3 TTTGTGTGAACAGGCGAGAA CCGACCGATTTCTTGAGTGT 45.0 50.0 58.0 57.9 1863 bp 0.5 59.1
ndhD_p4 TTGGAGTTGGGACCCAAAAG TGGTCCTGGCTCCTAAATCA 50.0 50.0 57.9 58.0 2458 bp 0.5 59.1
ndhD_p5 TTGGAGTTGGGACCCAAAAG GACCGAAGACTGTGTTGGTT 50.0 50.0 57.9 58.1 2188 bp 0.5 59.0