Hotspot

ccsA

SSC · 114887-115849 · 963 bp

Pi 0.475

MarkerSeek score 80.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 376
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCGTTTTCAAGGTGGGGAG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	2138 bp	0.5	59.6
ccsA_p2	TTCGTTTTCAAGGTGGGGAG	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	2137 bp	0.5	59.6
ccsA_p3	TTCGTTTTCAAGGTGGGGAG	TTCTCGCCTGTTCACACAAA	50.0	45.0	58.0	58.0	1613 bp	0.5	59.6
ccsA_p4	TTCGTTTTCAAGGTGGGGAG	TGGTGCTGCACTCTTTTTCT	50.0	45.0	58.0	57.9	2248 bp	0.5	59.3
ccsA_p5	TTCGTTTTCAAGGTGGGGAG	TTTTTGATTCTGGACCGCGA	50.0	45.0	58.0	58.1	1910 bp	0.5	58.9